homogeneously staining regions or double minute chromosomes in leukemia (brief communication)

Homogeneously Staining Regions or Double Minute Chromosomes in Leukemia (Brief Communication)

Identification of homogeneously staining regions in leukemia patients

Homogeneously staining regions (HSR) or double minute chromosomes (dmin) are autonomously replicating extra-chromosomal elements that are frequently associated with gene amplification in a variety of cancers. The diagnosis of leukemia patients was based on characterization of the leukemic cells obtained from bone marrow cytogenetics. This study report two cases, one with Acute Myeloblastic Leuk...

Double minute chromosomes in acute myeloblastic leukemia.

Two patients with acute myeloblastic leukemia (AML) with double minute chromosomes (dmins) are described. One patient had dmins in approximately one-third of bone marrow cells examined at diagnosis; no other karyotypic changes were observed. The dmins disappeared when the patient achieved a complete remission. The second patient developed acute leukemia as a second cancer, having previously rec...

identification of homogeneously staining regions in leukemia patients

homogeneously staining regions (hsr) or double minute chromosomes (dmin) are autonomously replicating extra-chromosomal elements that are frequently associated with gene amplifi cation in a variety of cancers. the diagnosis of leukemia patients was based on characterization of the leukemic cells obtained from bone marrow cytogenetics. this study report two cases, one with acute myeloblastic leu...

Genomic organization and evolution of double minutes/homogeneously staining regions with MYC amplification in human cancer

The mechanism for generating double minutes chromosomes (dmin) and homogeneously staining regions (hsr) in cancer is still poorly understood. Through an integrated approach combining next-generation sequencing, single nucleotide polymorphism array, fluorescent in situ hybridization and polymerase chain reaction-based techniques, we inferred the fine structure of MYC-containing dmin/hsr amplicon...

Homogeneously staining regions in direct preparations from human neuroblastomas.

Previous descriptions of homogeneously staining regions in human neuroblastomas have been in karyotypes obtained from established cell lines. We now report homogeneously staining regions in direct preparations from two human neuroblastomas. In one of the cases, the homogeneously staining region identified in the primary tumor was also found in metastases to bone marrow and pleural fluid. The ho...